MONOCLONAL-ANTIBODIES TO PURIFIED CUTINASE FROM FUSARIUM-SOLANI F SP PISI

A panel of murine monoclonal antibodies and polyclonal antibodies have been raised to purified cutinase from culture fluids of Fusarium solani f.sp. pisi, grown in a liquid medium containing purified apple cutin as the sole carbon source. All the antibodies recognized cutinase by both ELISA and Western blotting and three proved useful in immunofluorescence labelling studies. The polyclonal antiserum labelled hyphae, but not spores, of F. solani f.sp. pisi grown on cutin water agar. A similar, but weaker immunolabelling pattern, was observed with hyphae and spores of Botrytis cinerea. Cutinase was also detected by immunofluorescence on the surfaces of conidia of Erysiphe graminis var hordei and uredospores of Puccinia sp. © 1993 Academic Press, Inc.