THE SENSITIVITY OF EXPERIMENTS WHICH USE GRADIENT PULSES FOR COHERENCE-PATHWAY SELECTION

被引:88
作者
KONTAXIS, G
STONEHOUSE, J
LAUE, ED
KEELER, J
机构
[1] UNIV CAMBRIDGE,DEPT CHEM,CAMBRIDGE CB2 1EW,ENGLAND
[2] UNIV CAMBRIDGE,DEPT BIOCHEM,CAMBRIDGE CB2 1QP,ENGLAND
关键词
D O I
10.1006/jmra.1994.1227
中图分类号
O64 [物理化学(理论化学)、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号
070203 ; 070304 ; 081704 ; 1406 ;
摘要
The use of field-gradient pulses for coherence selection in multidimensional NMR experiments may cause a reduction in sensitivity per unit time when compared to experiments which use phase cycling. In addition, care must be taken to introduce the gradients in such a way as to retain the possibility of producing spectra with pure phase (i.e., absorption-mode lineshapes). The commonly used methods of implementing gradient pulses in multidimensional experiments are evaluated and compared with respect to these issues. These predictions are confirmed for a simple molecular system. The experimentally achieved sensitivities are also compared, on a residue-by-residue basis, for the case of different nitrogen-15 proton HSQC spectra of a 166 residue protein. (C) 1994 Academic Press, Inc.
引用
收藏
页码:70 / 76
页数:7
相关论文
共 22 条
[1]   SENSITIVITY OF 2-DIMENSIONAL NMR-SPECTROSCOPY [J].
AUE, WP ;
BACHMANN, P ;
WOKAUN, A ;
ERNST, RR .
JOURNAL OF MAGNETIC RESONANCE, 1978, 29 (03) :523-533
[2]   PHASE SEPARATION IN 2-DIMENSIONAL SPECTROSCOPY [J].
BACHMANN, P ;
AUE, WP ;
MULLER, L ;
ERNST, RR .
JOURNAL OF MAGNETIC RESONANCE, 1977, 28 (01) :29-39
[3]   OPTIMIZED RECORDING OF HETERONUCLEAR MULTIDIMENSIONAL NMR-SPECTRA USING PULSED FIELD GRADIENTS [J].
BAX, A ;
POCHAPSKY, SS .
JOURNAL OF MAGNETIC RESONANCE, 1992, 99 (03) :638-643
[4]   NATURAL ABUNDANCE N-15 NMR BY ENHANCED HETERONUCLEAR SPECTROSCOPY [J].
BODENHAUSEN, G ;
RUBEN, DJ .
CHEMICAL PHYSICS LETTERS, 1980, 69 (01) :185-189
[5]   SELECTION OF COHERENCE-TRANSFER PATHWAYS IN NMR PULSE EXPERIMENTS [J].
BODENHAUSEN, G ;
KOGLER, H ;
ERNST, RR .
JOURNAL OF MAGNETIC RESONANCE, 1984, 58 (03) :370-388
[6]   SENSITIVITY IMPROVEMENT IN ISOTROPIC MIXING (TOCSY) EXPERIMENTS [J].
CAVANAGH, J ;
RANCE, M .
JOURNAL OF MAGNETIC RESONANCE, 1990, 88 (01) :72-85
[7]   EXPERIMENTS FOR RECORDING PURE-ABSORPTION HETERONUCLEAR CORRELATION SPECTRA USING PULSED FIELD GRADIENTS [J].
DAVIS, AL ;
KEELER, J ;
LAUE, ED ;
MOSKAU, D .
JOURNAL OF MAGNETIC RESONANCE, 1992, 98 (01) :207-216
[8]  
DROBNY G, 1979, FARADAY DIV CHEM SOC, V13, P49
[9]  
Ernst RR, 1987, PRINCIPLES NUCL MAGN
[10]   GRADIENT-ENHANCED SPECTROSCOPY [J].
HURD, RE .
JOURNAL OF MAGNETIC RESONANCE, 1990, 87 (02) :422-428