ACTIVATION OF C-KI-RAS NOT DETECTABLE IN ADENOMAS OR ADENOCARCINOMAS ARISING IN RAT PANCREAS

被引：38

作者：

SCHAEFFER, BK ^{[1
]}

ZURLO, J ^{[1
]}

LONGNECKER, DS ^{[1
]}

机构：

[1] DARTMOUTH COLL, HITCHCOCK MED CTR, DARTMOUTH MED SCH, DEPT PHARMACOL & TOXICOL, HANOVER, NH 03756 USA

来源：

MOLECULAR CARCINOGENESIS | 1990年 / 3卷 / 03期

关键词：

Key words; mutation; oncogene; Polymerase chain reaction;

D O I：

10.1002/mc.2940030310

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The objective of this study was to determine whether activation of c‐Ki‐ras occurs in carcinogen‐induced rat pancreatic tumors. DNAs from 27 samples, which included adenomas, carcinomas in situ, and adenocarcinomas arising in azaserine‐treated rats and corn oil‐gavaged rats along with a nafenopin‐induced rat pancreatic adenocarcinoma were examined for mutation of c‐Ki‐ras at codons 12, 13, and 61 by using the polymerase chain reaction. Our results indicate that activation of c‐Ki‐ras is not a common event during pancreatic carcinogenesis in the rat. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：165 / 170

页数：6

共 30 条

[1] MOST HUMAN CARCINOMAS OF THE EXOCRINE PANCREAS CONTAIN MUTANT C-K-RAS GENES
ALMOGUERA, C
SHIBATA, D
FORRESTER, K
MARTIN, J
ARNHEIM, N
PERUCHO, M
[J]. CELL, 1988, 53 (04) : 549 - 554
[2] ACTIVATION OF THE MOUSE CELLULAR HARVEY-RAS GENE IN CHEMICALLY-INDUCED BENIGN SKIN PAPILLOMAS
BALMAIN, A
RAMSDEN, M
BOWDEN, GT
SMITH, J
[J]. NATURE, 1984, 307 (5952) : 658 - 660
[3] PREVALENCE OF RAS GENE-MUTATIONS IN HUMAN COLORECTAL CANCERS
BOS, JL
FEARON, ER
HAMILTON, SR
VERLAANDEVRIES, M
VANBOOM, JH
VANDEREB, AJ
VOGELSTEIN, B
[J]. NATURE, 1987, 327 (6120) : 293 - 297
[4] ACTIVATION OF KI-RAS 2 GENE IN HUMAN-COLON AND LUNG CARCINOMAS BY 2 DIFFERENT POINT MUTATIONS
CAPON, DJ
SEEBURG, PH
MCGRATH, JP
HAYFLICK, JS
EDMAN, U
LEVINSON, AD
GOEDDEL, DV
[J]. NATURE, 1983, 304 (5926) : 507 - 513
[5] CUBILLA AL, 1980, PANCREAS, P82
[6] DETECTION OF A CELLULAR ONCOGENE IN SPONTANEOUS LIVER-TUMORS OF B6C3F1 MICE
FOX, TR
WATANABE, PG
[J]. SCIENCE, 1985, 228 (4699) : 596 - 597
[7] STRUCTURE AND EXPRESSION OF AMPLIFIED CKI-RAS GENE-SEQUENCES IN Y1 MOUSE ADRENAL-TUMOR CELLS
GEORGE, DL
SCOTT, AF
TRUSKO, S
GLICK, B
FORD, E
DORNEY, DJ
[J]. EMBO JOURNAL, 1985, 4 (05) : 1199 - 1203
[8] HIGH-FREQUENCY OF KI-RAS CODON-12 MUTATIONS IN PANCREATIC ADENOCARCINOMAS
GRUNEWALD, K
LYONS, J
FROHLICH, A
FEICHTINGER, H
WEGER, RA
SCHWAB, G
JANSSEN, JWG
BARTRAM, CR
[J]. INTERNATIONAL JOURNAL OF CANCER, 1989, 43 (06) : 1037 - 1041
[9] GU JR, 1986, J CELL PHYSIOL, P13
[10] ACTIVATION OF THE C-K-RAS ONCOGENE IN A HUMAN-PANCREAS CARCINOMA
HIRAI, H
OKABE, T
ANRAKU, Y
FUJISAWA, M
URABE, A
TAKAKU, F
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 127 (01) : 168 - 174

← 1 2 3 →