A useful and rapid method to recover bacterial cells from milk samples for microscopic count

被引：1

作者：

FernandezAstorga, A ^{[1
]}

Hijarrubia, MJ ^{[1
]}

Lazaro, B ^{[1
]}

Barcina, I ^{[1
]}

机构：

[1] UNIV BASQUE COUNTRY, DEPT MICROBIOL IMMUNOL & PARASITOL, FAC CIENCIAS, E-48080 BILBAO, SPAIN

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1995年 / 24卷 / 02期

关键词：

bacterial recovery; direct epifluorescent filter enumeration (DEFT count); milk pre-treatment;

D O I：

10.1016/0167-7012(95)00060-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A description is given of a pre-treatment for milk samples which allows it to be filtered through 0.2 mu m pore-size membrane filters without reducing the initial number of bacteria. The efficiency of the method was demonstrated in milk samples inoculated with Escherichia coli O25:H-42, a food-borne pathogen, by comparison with a control in tryptone soya broth (TSB). No significant differences were found between direct microscopic counts in milk samples and controls in TSB.

引用

页码：111 / 115

页数：5

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