SELECTIVE FLOCCULATION WITH CHITOSAN IN ESCHERICHIA-COLI DISINTEGRATES - EFFECTS OF IONIC-STRENGTH AND MULTIVALENT METAL-IONS

被引：10

作者：

AGERKVIST, I

ERIKSSON, LB

机构：

[1] Institute for Surface Chemistry, S-114 86 Stockholm

来源：

JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY | 1993年 / 14卷 / 03期

关键词：

D O I：

10.1080/01932699308943404

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The effects of ionic strength and multivalent metal ions on the flocculation performance in Escherichia coli cell disintegrates using the polycationic chitosan as a flocculant were investigated.The study showed that ionic strength could be used to optimize the selectivity in flocculation. The best selectivity was obtained at 0.5 M sodium chloride where 95% of the cell debris and 88% of the nucleic acids could be removed from the disintegrate by sedimentation, leaving 99% of proteins and 86% of 8-galactosidase enzyme activity in clarified solution. We will also discuss the mechanisms for interaction between chitosan and different groups of components in the disintegrate: cell debris particles, nucleic acid polymers and proteins.

引用

页码：269 / 293

页数：25

共 35 条

[1] SELECTIVE FLOCCULATION WITH CHITOSAN IN ESCHERICHIA-COLI DISINTEGRATES - EFFECTS OF PH AND NUCLEASE TREATMENT [J].