PURIFICATION AND PROPERTIES OF THE ENDOCELLULAR BETA-GLUCOSIDASE OF CANDIDA-CACAOI BUCKLEY AND VANUDEN CBS 2020

被引：13

作者：

DRIDER, D ^{[1
]}

POMMARES, P ^{[1
]}

CHEMARDIN, P ^{[1
]}

ARNAUD, A ^{[1
]}

GALZY, P ^{[1
]}

机构：

[1] ECOLE NATL SUPER AGRON MONTPELLIER,CHAIRE MICROBIOL IND & GENET MICROORGANISMES,PL P VIALA,F-34070 MONTPELLIER 01,FRANCE

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1993年 / 74卷 / 04期

关键词：

D O I：

10.1111/j.1365-2672.1993.tb05156.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The endocellular enzyme beta-glucosidase of Candida cacaoi was purified by ion-exchange chromatography and gel filtration. The molecular weight was 220 +/- 10 kDa; its optimum pH was between 4 and 5.5 and its optimum temperature was 60-degrees-C. This enzyme was active against soluble glucosides tested with beta(1-2), beta(1-3), beta(1-4) and even alpha(1-4) and alpha(1-6) and was inhibited by D-glucono-delta-lactone. The enzyme was constitutive but its synthesis was repressed by glucose.

引用

页码：473 / 479

页数：7

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