CONDENSATION OF SUPERCOILED DNA INDUCED BY MNCL2

被引：124

作者：

MA, CL ^{[1
]}

BLOOMFIELD, VA ^{[1
]}

机构：

[1] UNIV MINNESOTA,DEPT BIOCHEM,ST PAUL,MN 55108

来源：

BIOPHYSICAL JOURNAL | 1994年 / 67卷 / 04期

关键词：

D O I：

10.1016/S0006-3495(94)80641-1

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Multivalent cations condense DNA in vitro, but it had been thought that a valence of at least +3 was required in aqueous solution. We have found that Mn2+ can produce toroidal condensates of supercoiled plasmid DNA, but not of linearized plasmid. Mg2+ does not cause condensation, and neither MgCl2 nor NaCl can negate the effect of MnCl2, indicating that the condensation mechanism with Mn is not primarily electrostatic. Supercoiled MnDNA is more extensively digested than the linear form by S1 nuclease. Supercoiling appears to cooperate with Mn2+ in stabilizing helix distortions and also provides a ''pressure'' that enhances lateral association.

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页码：1678 / 1681

页数：4

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