IMMOBILIZATION OF RHODOCCUS-EQUI DSM 89-133 ONTO POROUS CELITE BEADS FOR CHOLESTEROL SIDE-CHAIN CLEAVAGE

被引：12

作者：

AHMAD, S ^{[1
]}

JOHRI, BN ^{[1
]}

机构：

[1] GOVIND BALLABH PANT UNIV AGR & TECHNOL,DEPT BASIC SCI & HUMANITIES,PANTNAGAR 263145,UTTAR PRADESH,INDIA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1992年 / 37卷 / 04期

关键词：

D O I：

10.1007/BF00180970

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rhodococcus equi cells immobilized onto porous celite beads were active in side-chain cleavage of cholesterol. The effect of bead size, adsorption time and washing cycles were examined. The degradation of cholesterol side-chain using adsorbed cells was studied in batch and semi-continuous systems. Compared to free cells (80 mol %), the end-product (1,4-androstadiene-3,17-dione and 4-androstene-3,17-dione) profile was lower (70 mol %) with the celite-adsorbed system.

引用

页码：468 / 469

页数：2

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