IDENTIFICATION OF A SET OF RFLP PROBES FOR SUBSPECIES DIFFERENTIATION IN ORYZA-SATIVA L

被引：26

作者：

QIAN, HR ^{[1
]}

ZHUANG, JY ^{[1
]}

LIN, HX ^{[1
]}

LU, J ^{[1
]}

ZHENG, KL ^{[1
]}

机构：

[1] CHINA NATL RICE RES INST,DEPT BIOTECHNOL,HANGZHOU 310006,PEOPLES R CHINA

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 90卷 / 06期

关键词：

RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP); PROBE SCREENING; SUBSPECIES DIFFERENTIATION; WIDE COMPATIBILITY VARIETY; ORYZA SATIVA L;

D O I：

10.1007/BF00222026

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Sixty-eight indica-japonica tester-differentiating RFLP probes were tested in seven indica and seven japonica varieties of rice (Orysa sativa L.) with four enzyme digestions (EcoRI, EcoRV, HindIII and DraI). Twenty-one DNA clones were isolated as indica-japonica subspecies-differentiating probes. A set of 13 probes was established as core probes for subspecies differentiation and a pooled blotting analysis was carried out to facilitate the application of RFLP in rice genetics and breeding practice. A dendrogram of 12 wide-compatibility varieties was constructed based on RFLPs detected by 13 core probes with single enzyme digestions. It was speculated that most RFLPs of indica-japonica differentiating probes were generated by insertions/deletions, which may be of great significance for the origin and differentiation of subspecies in Oryza sativa L.

引用

页码：878 / 884

页数：7

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