IDENTIFICATION OF A SET OF RFLP PROBES FOR SUBSPECIES DIFFERENTIATION IN ORYZA-SATIVA L

被引：26

作者：

QIAN, HR ^{[1
]}

ZHUANG, JY ^{[1
]}

LIN, HX ^{[1
]}

LU, J ^{[1
]}

ZHENG, KL ^{[1
]}

机构：

[1] CHINA NATL RICE RES INST,DEPT BIOTECHNOL,HANGZHOU 310006,PEOPLES R CHINA

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 90卷 / 06期

关键词：

RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP); PROBE SCREENING; SUBSPECIES DIFFERENTIATION; WIDE COMPATIBILITY VARIETY; ORYZA SATIVA L;

D O I：

10.1007/BF00222026

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Sixty-eight indica-japonica tester-differentiating RFLP probes were tested in seven indica and seven japonica varieties of rice (Orysa sativa L.) with four enzyme digestions (EcoRI, EcoRV, HindIII and DraI). Twenty-one DNA clones were isolated as indica-japonica subspecies-differentiating probes. A set of 13 probes was established as core probes for subspecies differentiation and a pooled blotting analysis was carried out to facilitate the application of RFLP in rice genetics and breeding practice. A dendrogram of 12 wide-compatibility varieties was constructed based on RFLPs detected by 13 core probes with single enzyme digestions. It was speculated that most RFLPs of indica-japonica differentiating probes were generated by insertions/deletions, which may be of great significance for the origin and differentiation of subspecies in Oryza sativa L.

引用

页码：878 / 884

页数：7

共 22 条

[11]

Oba S., Hanzawa M., Uchimiya H., Estimates of chromosome recombination by RFLPs and search for indica-specific DNA probes, Proc 3rd Wkshp Rice Mol Biol, August 20–22, 1991, (1991)

[12]

Oka H.I., Genetic diversity of wild and cultivated rice, Rice biotechnology, pp. 55-81, (1992)

[13]

Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A., Allard R.W., Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics, Proc Natl Acad Sci USA, 81, pp. 8014-8018, (1984)

[14]

Sokal R.R., Michener C.D., A statistical method for evaluating systematic relationships, Univ Kans Sci Bull, 28, pp. 1409-1438, (1958)

[15]

Tanksley S.D., Young N.D., Paterson A.H., Bonierbale M.W., RFLP mapping in plant breeding — new tools for an old science, Bio/Technology, 7, pp. 257-264, (1989)

[16]

Wang Z.Y., Tanksley S.D., Restriction fragment length polymorphism in Oryza sativa L, Genome, 32, pp. 1113-1118, (1989)

[17]

Wilimzig, LiC1-boiling method of plasmid mini-preps, Trends Genet, 1, (1985)

[18]

Yuan L.P., Breeding strategies for hybrid rice, Hybrid Rice, 1, pp. 1-3, (1987)

[19]

Zeng S., Yang X., Lu Z., The heterosis of F<sub>1</sub> hybrids between Oryza sativa subsp hsien and Oryza sativa subbsp keng, Acta Agro Sin, 6, pp. 145-150, (1980)

[20]

Zheng K., Shen B., Yu F., Zhao C., Qi X., Xu X., Restriction fragment length polymorphism in rice, Chinese J Rice Sci, 4, pp. 145-149, (1990)

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