A SIMPLE METHOD FOR CALCULATING THE DISSOCIATION-CONSTANT OF A RECEPTOR (OR ENZYME).UNLABELED LIGAND COMPLEX FROM RADIOLIGAND DISPLACEMENT MEASUREMENTS

被引：16

作者：

MARTIN, RL ^{[1
]}

RENOSTO, F ^{[1
]}

SEGEL, IH ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT BIOCHEM & BIOPHYS,BERKELEY,CA 94720

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1991年 / 284卷 / 01期

关键词：

D O I：

10.1016/0003-9861(91)90257-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A general procedure is described for determining the dissociation constant of a receptor (or enzyme) · unlabeled ligand complex (EI) by analyzing the I-dependent displacement of bound radioligand (A*) from EA*. The procedure (which involves measuring free A* in the presence of I) requires a knowledge of the total concentrations of receptor ([E]t), unlabeled ligand ([I]t), and radioligand ([A*]t), and the dissociation constant of the EA* complex. The unknown Kd is obtained from five simple, sequential calculations which are valid for either high or low affinity competitive unlabeled ligands and are independent of total receptor concentration or initial degree of saturation with A*. The procedure also provides the information needed to construct a distribution curve of all enzyme and ligand species (E, EA*, EI, A*, I) as [I]t is varied. © 1991.

引用

页码：26 / 29

页数：4

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