CALPASTATIN AND NUCLEOTIDES STABILIZE CARDIAC CALCIUM-CHANNEL ACTIVITY IN EXCISED PATCHES

被引：61

作者：

ROMANIN, C

GROSSWAGEN, P

SCHINDLER, H

机构：

[1] Institute for Biophysics, University of Linz, Linz, A-4040

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1991年 / 418卷 / 1-2期

关键词：

CALPASTATIN; CALPAIN; L-TYPE CALCIUM CHANNEL; PATCH CLAMP; PROTEASE INHIBITOR; RUNDOWN;

D O I：

10.1007/BF00370456

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The activity of single L-type Ca2+ channels is rapidly lost (run-down) when contact between the membrane and cytosol is interrupted. We have now achieved the stabilization of cardiac Ca2+ channel activity of guinea-pig ventricular myocytes by using either cytosol or defined components added to excised patches. The endogenous protease inhibitor, calpastatin, together with nucleotides, ATP + GTP, was found to prevent run-down as effectively as cardiac cytosolic solution. These results suggest the involvement of proteolysis by calpain in run-down of channel activity and enable the study of cardiac Ca2+ channel regulation with free access to both sides of the membrane.

引用

收藏

页码：86 / 92

页数：7

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