IRE1 ENCODES A PUTATIVE PROTEIN-KINASE CONTAINING A MEMBRANE-SPANNING DOMAIN AND IS REQUIRED FOR INOSITOL PHOTOTROPHY IN SACCHAROMYCES-CEREVISIAE

被引：161

作者：

NIKAWA, JI ^{[1
]}

YAMASHITA, S ^{[1
]}

机构：

[1] GUNMA UNIV,SCH MED,DEPT BIOCHEM,MAEBASHI,GUNMA 371,JAPAN

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 11期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb00864.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel gene, IRE1, of Saccharomyces cerevisiae was cloned through genetic complementation of a myo-inositol auxotrophic mutant. The predicted amino acid sequence indicated that IRE1 encodes a protein of 126983Da with two highly hydrophobic regions, probably a signal sequence and a membrane-spanning region. The carboxy-terminal region of IRE1 showed close sequence similarity to the catalytic domains of protein kinases. Disruption of the IRE1 locus caused myo-inositol auxotrophy. The IRE1 product is very likely a protein kinase required for myo-inositol synthesis.

引用

页码：1441 / 1446

页数：6

共 20 条

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