INTRACELLULAR DISTRIBUTION AND SITES OF SYNTHESIS OF GLYCOSAMINOGLYCANS(MUCOPOLYSACCHARIDES) IN HUMAN LEUKOCYTES

被引：36

作者：

OLSSON, I

机构：

[1] Department of Physiological Chemistry, Research Laboratory of the Hospital, University of Lund, Lund

来源：

EXPERIMENTAL CELL RESEARCH | 1969年 / 54卷 / 03期

关键词：

D O I：

10.1016/0014-4827(69)90208-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The content of chondroitin sulfate was measured in leukocyte subcellular fractions prepared by differential centrifugation and density gradient centrifugation. The granule fraction contained the bulk of chondroitin sulfate while the microsomal fraction contained only small amounts of this polysaccharide. The biosynthesis of chondroitin sulfate was localized to microsomal structures since these exhibited the highest specific activity after incubation of the cells with 35S-sulfate and 14C-glucosamine. The occurrence in the leukocyte granules of ionic interactions between chondroitin sulfate and other substances is indicated as NaCl of high ionic strength was needed for release from the granules of the polysaccharide. © 1969.

引用

页码：314 / &

共 16 条

[1] DETERMINATION OF GLYCOSAMINOGLYCANS ( MUCOPOLYSACCHARIDES ) FROM TISSUES ON MICROGRAM SCALE [J].