CNTF PROMOTES THE SURVIVAL OF NEONATAL RAT CORTICOSPINAL NEURONS INVITRO

被引:35
作者
MAGAL, E
LOUIS, JC
OUDEGA, M
VARON, S
机构
[1] Department of Biology, University of California, San Diego, La Jolla, CA
关键词
CORTICOSPINAL NEURON; NEURONAL SURVIVAL; CELL CULTURE; CNTF;
D O I
10.1097/00001756-199306000-00046
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
CORTICOSPINAL neurons were identified in cell cultures of neonatal rat cortex by immunostaining of cholera toxin B subunit (CTB), retrogradely transported from the cervical part of the spinal cord. The CTB-immunoreactive neurons were larger than the neurons in the overall (unstained) neuronal population and represented a small fraction of it (average of 0.3%) after 6 hours in vitro. The number of both total and CTB-labeled neurons declined progressively with time in culture. The neuronal death was, however, markedly faster in the CTB-labeled neuronal population than in the overall neuronal population. Ciliary neurotrophic factor (CNTF) promoted the survival of CTB-positive corticospinal neurons in a dose-dependent manner; with CNTF, the death rate of the CTB-labeled neurons became identical to that of the overall population.
引用
收藏
页码:779 / 782
页数:4
相关论文
共 17 条
  • [11] CNTF PROTECTION OF OLIGODENDROCYTES AGAINST NATURAL AND TUMOR NECROSIS FACTOR-INDUCED DEATH
    LOUIS, JC
    MAGAL, E
    TAKAYAMA, S
    VARON, S
    [J]. SCIENCE, 1993, 259 (5095) : 689 - 692
  • [12] EFFECTS OF CILIARY NEURONOTROPHIC FACTOR ON RAT SPINAL-CORD NEURONS INVITRO - SURVIVAL AND EXPRESSION OF CHOLINE-ACETYLTRANSFERASE AND LOW-AFFINITY NERVE GROWTH-FACTOR RECEPTORS
    MAGAL, E
    BURNHAM, P
    VARON, S
    [J]. DEVELOPMENTAL BRAIN RESEARCH, 1991, 63 (1-2): : 141 - 150
  • [13] Manthorpe M, 1993, NEUROTROPHIC FACTORS, P443
  • [14] CONTROL OF EMBRYONIC MOTONEURON SURVIVAL INVIVO BY CILIARY NEUROTROPHIC FACTOR
    OPPENHEIM, RW
    PREVETTE, D
    YIN, QW
    COLLINS, F
    MACDONALD, J
    [J]. SCIENCE, 1991, 251 (5001) : 1616 - 1618
  • [15] CILIARY NEUROTROPHIC FACTOR PREVENTS DEGENERATION OF MOTOR NEURONS IN MOUSE MUTANT PROGRESSIVE MOTOR NEURONOPATHY
    SENDTNER, M
    SCHMALBRUCH, H
    STOCKLI, KA
    CARROLL, P
    KREUTZBERG, GW
    THOENEN, H
    [J]. NATURE, 1992, 358 (6386) : 502 - 504
  • [16] CILIARY NEUROTROPHIC FACTOR PREVENTS THE DEGENERATION OF MOTOR NEURONS AFTER AXOTOMY
    SENDTNER, M
    KREUTZBERG, GW
    THOENEN, H
    [J]. NATURE, 1990, 345 (6274) : 440 - 441
  • [17] THE DEVELOPMENT OF THE CORTICOSPINAL PROJECTION
    STANFIELD, BB
    [J]. PROGRESS IN NEUROBIOLOGY, 1992, 38 (02) : 169 - 202