PRIMARY STRUCTURE OF 10 GALACTOSIDES FORMED BY TRANSGLYCOSYLATION DURING LACTOSE HYDROLYSIS BY BIFIDOBACTERIUM-BIFIDUM

Oligosaccharides formed by a transgalactosylation reaction during lactose hydrolysis with Bifidobacterium bifidum were separated into eight fractions by gel-permeation chromatography and their structures studies determined by trimethylsilylation analysis, methylation analysis, f.a.b.-m.s., g.l.c.-m.s. and enzymic hydrolysis as β-d-Galp-(1→3)-d-Glc, β-d-Galp-(1→6)-d-Glc, β-d-Galp-(1→6)-d-Glc, β-d-Galp-(1→3)-β-d-Galp-(1→4)-d-Glc, β-d-Galp-(1→6)[β-d-Galp-(1→4)]-d-Glc, β-d-Galp-(1→2)[β-d-Galp-(1→6)]-d-Glc, β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→4)-d-Glc, β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→4)-d-Glc, β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-dGalp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→4)-d-Glc, and β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→3) β-d-Galp-(1→4)-d-Glc. © 1990.