IMPORTANCE OF THIOLS IN THE REDUCTIVE BINDING OF 2-NITROIMIDAZOLES TO MACROMOLECULES

被引：104

作者：

RALEIGH, JA ^{[1
]}

KOCH, CJ ^{[1
]}

机构：

[1] UNIV PENN,DEPT RADIAT ONCOL,PHILADELPHIA,PA 19104

来源：

BIOCHEMICAL PHARMACOLOGY | 1990年 / 40卷 / 11期

关键词：

D O I：

10.1016/0006-2952(90)90086-Z

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Reductive activation of 2-nitroimidazoles in the presence of bovine serum albumin (BSA) led to binding of the nitroheterocycles to the protein. The binding was most efficient to BSA in which protein disulfides had been reduced to thiol groups. Protein thiols were at least twenty times more efficient than other protein, RNA or DNA nucleophiles in binding the reductively-activated nitroheterocycles. This result is of practical importance in the development of immunoassays for 2-nitroimidazoles as hypoxia markers in normal and tumor tissue. © 1990.

引用

页码：2457 / 2464

页数：8

共 53 条

[11] STUDIES ON FORMATION OF PRIMARY YIELDS OF HYDROXYL RADICAL AND HYDRATED ELECTRON IN GAMMA-RADIOLYSIS OF WATER [J].