REEXAMINATION OF THE ROLE OF ASP20 IN CATALYSIS BY BACTERIOPHAGE-T4 LYSOZYME

被引：51

作者：

HARDY, LW ^{[1
]}

POTEETE, AR ^{[1
]}

机构：

[1] UNIV MASSACHUSETTS,DEPT MOLEC GENET & MICROBIOL,WORCESTER,MA 01655

来源：

BIOCHEMISTRY | 1991年 / 30卷 / 39期

关键词：

D O I：

10.1021/bi00103a010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Replacement of Asp20 in T4 lysozyme by Cys produces a variant with (1) nearly wild-type specific activity, (2) a newly acquired sensitivity to thiol-modifying reagents, and (3) a pH-activity profile that is very similar to that of the wild-type enzyme. These results indicate that the residue at position 20 has a significant nucleophilic function rather than merely an electrostatic role. The intermediate in catalysis by lysozyme is probably a covalent glycosyl-enzyme instead of the ion pair originally proposed.

引用

页码：9457 / 9463

页数：7

共 53 条

[21]

JENSEN H B, 1976, European Journal of Biochemistry, V66, P319, DOI 10.1111/j.1432-1033.1976.tb10521.x

[22] X-RAY CRYSTALLOGRAPHY OF THE BINDING OF THE BACTERIAL-CELL WALL TRISACCHARIDE NAM-NAG-NAM TO LYSOZYME [J].

KELLY, JA ;

SIELECKI, AR ;

SYKES, BD ;

JAMES, MNG ;

PHILLIPS, DC .

NATURE, 1979, 282 (5741) :875-878

[23] MECHANISM AND STEREOELECTRONIC EFFECTS IN THE LYSOZYME REACTION [J].

KIRBY, AJ .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1987, 22 (04) :283-315

[24] MECHANISM OF REACTIONS OF N-(METHOXYMETHYL)-N,N-DIMETHYLANILINIUM IONS WITH NUCLEOPHILIC-REAGENTS [J].

KNIER, BL ;

JENCKS, WP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1980, 102 (22) :6789-6798

[25] MUTATIONS IN AN UPSTREAM REGULATORY SEQUENCE THAT INCREASE EXPRESSION OF THE BACTERIOPHAGE-T4 LYSOZYME GENE [J].

KNIGHT, JA ;

HARDY, LW ;

RENNELL, D ;

HERRICK, D ;

POTEETE, AR .

JOURNAL OF BACTERIOLOGY, 1987, 169 (10) :4630-4636

[26] STEREOCHEMISTRY AND THE MECHANISM OF ENZYMATIC REACTIONS [J].

KOSHLAND, DE .

BIOLOGICAL REVIEWS OF THE CAMBRIDGE PHILOSOPHICAL SOCIETY, 1953, 28 (04) :416-436

[27]

KUROKI R, 1986, J BIOL CHEM, V261, P3571

[28] EXTRACTION OF NUCLEIC-ACIDS FROM AGAROSE GELS [J].

LANGRIDGE, J ;

LANGRIDGE, P ;

BERGQUIST, PL .

ANALYTICAL BIOCHEMISTRY, 1980, 103 (02) :264-271

[29] ELECTROSTATIC FACILITATION OF GENERAL ACID-CATALYZED ALPHA-OXONIUM ION FORMATION IN A LYSOZYME-LIKE ENVIRONMENT - KINETIC INVESTIGATIONS [J].

LOUDON, GM ;

RYONO, DE .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1976, 98 (07) :1900-1907

[30] SITE-DIRECTED MUTAGENESIS OF THE CATALYTIC RESIDUES ASP-52 AND GLU-35 OF CHICKEN EGG-WHITE LYSOZYME [J].

MALCOLM, BA ;

ROSENBERG, S ;

COREY, MJ ;

ALLEN, JS ;

DEBAETSELIER, A ;

KIRSCH, JF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (01) :133-137

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