DNA-SEQUENCING WITH A HEXAMER STRING PRIMER AND DYE-LABELED TERMINATORS

被引：9

作者：

HOU, WY ^{[1
]}

SMITH, LM ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 221卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1389

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for fluorescence-based DNA sequencing using a hexamer string primer and dye-labeled terminators is described. Sequencing protocols have been determined that provide an average read length of 393 bases with 99.6% base-calling accuracy for single-stranded M13mp18 DNA, and an average read length of 367 bases with 99.4% base-calling accuracy for double-stranded M13mp19 DNA. The signal intensity is stronger than that obtained with a standard 18-mer primer, while the resolution and length of readable sequence are comparable. (C) 1994 Academic Press, Inc.

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页码：136 / 141

页数：6

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