THE ISOLATION OF LYTIC ENZYMES FROM CYTOPHAGA AND THEIR APPLICATION TO THE RUPTURE OF YEAST-CELLS

被引：18

作者：

ASENJO, JA

DUNNILL, P

机构：

[1] UNIV CHILE, ESCUELA & INGN, SANTIAGO, CHILE

[2] UNIV LONDON UNIV COLL, DEPT CHEM & BIOCHEM ENGN, LONDON WC1E 7JE, ENGLAND

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1981年 / 23卷 / 05期

关键词：

Enzymes; -; Precipitation; (chemical);

D O I：

10.1002/bit.260230512

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Yeast-lytic enzymes were isolated on a pilot scale from Cytophaga spp. by precipitation and the light, enzyme-rich solid phase recovered by liquid-liquid separation. The enzyme complex was immobilized to soluble polymeric carbohydrates and the effectiveness of the free and immobilized enzyme for protein release and cell debris dissolution was assessed.

引用

页码：1045 / 1056

页数：12

共 21 条

[11]

GUTFREUND H, 1975, ENZYMES PHYSICAL PRI

[12] NON-POROUS MAGNETIC SUPPORTS FOR PROTEASES, CELL-LYTIC ENZYMES, AND RIBONUCLEASE - LIMITS OF REACTANT SIZE [J].