AORTIC ENDOTHELIAL-CELL DAMAGE INDUCED BY BETA-VLDL AND MACROPHAGES INVITRO

被引：12

作者：

TANIMURA, N ^{[1
]}

ASADA, Y ^{[1
]}

HAYASHI, T ^{[1
]}

KISANUKI, A ^{[1
]}

SUMIYOSHI, A ^{[1
]}

机构：

[1] MIYAZAKI MED COLL,DEPT PATHOL 1,5200 KIHARA,MIYAZAKI 88916,JAPAN

来源：

ATHEROSCLEROSIS | 1990年 / 85卷 / 2-3期

关键词：

AORTIC ENDOTHELIAL CELL INJURY; BETA-VLDL; MONOCYTE MACROPHAGE; ANTIOXIDANTS;

D O I：

10.1016/0021-9150(90)90107-T

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We performed an in vitro study to assess damage to swine aortic endothelial cells by rabbit beta-VLDL and/or rabbit peritoneal macrophages. Incubation of cultured aortic endothelial cells with beta-VLDL, macrophages, or macrophage lysate induced endothelial cell damage time- and dose-dependently as estimated by [H-3]adenine release. Incubation of endothelial cells with both beta-VLDL and macrophages produced a synergistic effect on the increase of [H-3]adenine release. Pretreatment of the endothelial cells with some kinds of antioxidants (probucol 560-mu-g/ml, vitamin E 50-mu-M, superoxide dismutase-polyethylene glycol 0.5 mg/ml, or catalase-polyethylene glycol 0.5-1.0 mg/ml) significantly prevented the endothelial damage by beta-VLDL or macrophage lysate. We conclude that beta-VLDL and/or macrophages could induce endothelial cell damage and that some kinds of antioxidants could prevent it.

引用

页码：161 / 167

页数：7

共 21 条

[1]

ALDERSON LM, 1986, AM J PATHOL, V123, P334

[2]

BECKMAN JS, 1988, J BIOL CHEM, V263, P6884

[3]

ENDEMANN G, 1987, AM J PATHOL, V126, P1

[4] CHEMICALLY MODIFIED LOW-DENSITY LIPOPROTEINS AS INDUCERS OF ENZYME-RELEASE FROM MACROPHAGES [J].

HARTUNG, HP ;

KLADETZKY, RG ;

HENNERICI, M .

FEBS LETTERS, 1985, 186 (02) :211-214

[5]

HARTUNG HP, 1986, LAB INVEST, V55, P209

[6] LIPOPROTEIN OXIDATION AND LIPOPROTEIN-INDUCED CYTO-TOXICITY [J].

HESSLER, JR ;

MOREL, DW ;

LEWIS, LJ ;

CHISOLM, GM .

ARTERIOSCLEROSIS, 1983, 3 (03) :215-222

[7] INVITRO STUDY OF VASCULAR ENDOTHELIAL INJURY BY ACTIVATED PLATELETS AND ITS PREVENTION [J].

KISHI, Y ;

NUMANO, F .

ATHEROSCLEROSIS, 1989, 76 (2-3) :95-101

[8] PROBUCOL PREVENTS THE PROGRESSION OF ATHEROSCLEROSIS IN WATANABE HERITABLE HYPERLIPIDEMIC RABBIT, AN ANIMAL-MODEL FOR FAMILIAL HYPERCHOLESTEROLEMIA [J].

KITA, T ;

NAGANO, Y ;

YOKODE, M ;

ISHII, K ;

KUME, N ;

OOSHIMA, A ;

YOSHIDA, H ;

KAWAI, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (16) :5928-5931

[9]

MAHLEY RW, 1980, J LIPID RES, V21, P970

[10]

MOREL DW, 1983, J LIPID RES, V24, P1070

← 1 2 3 →