YEAST ARTIFICIAL CHROMOSOME VECTORS FOR EFFICIENT CLONE MANIPULATION AND MAPPING

被引：33

作者：

SHERO, JH

MCCORMICK, MK

ANTONARAKIS, SE

HIETER, P

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT MOLEC BIOL & GENET,BALTIMORE,MD 21205

[2] JOHNS HOPKINS UNIV,SCH MED,CTR MED GENET,DEPT PEDIAT,BALTIMORE,MD 21205

来源：

GENOMICS | 1991年 / 10卷 / 02期

关键词：

D O I：

10.1016/0888-7543(91)90343-D

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The yeast artificial chromosome (YAC) cloning system allows the cloning of exogenous DNA several hundred kilobases in length. To enhance the usefulness of this technology, yeast artificial chromosome vectors have been designed for efficient clone characterization, manipulation, and mapping. The vectors contain a polylinker with unique EcoRI, BglII, NotI, EagI, SacII, SalI, NruI, NheI, and ClaI cloning sites and T7 bacteriophage promoters positioned to allow the generation of riboprobes from the exogenous DNA ends. Centric and acentric vector arms were constructed as separate plasmids to allow the recovery of both ends of the YAC insert DNA directly in Escherichia coli. In addition, YACs generated using this vector system contain a yeast gene (SUP11) that allows visual monitoring of YAC stability and copy number. © 1991.

引用

页码：505 / 508

页数：4

共 14 条

[11]

SIKORSKI RS, 1989, GENETICS, V122, P19

[12] PHYSICAL MAPPING OF LARGE DNA BY CHROMOSOME FRAGMENTATION [J].