HIGH-RESOLUTION SEPARATION OF PCR PRODUCT AND GENE DIAGNOSIS BY CAPILLARY GEL-ELECTROPHORESIS

被引：24

作者：

BABA, Y ^{[1
]}

TOMISAKI, R ^{[1
]}

SUMITA, C ^{[1
]}

TSUHAKO, M ^{[1
]}

MIKI, T ^{[1
]}

OGIHARA, T ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT GERIATR MED,SUITA,OSAKA 565,JAPAN

来源：

BIOMEDICAL CHROMATOGRAPHY | 1994年 / 8卷 / 06期

关键词：

D O I：

10.1002/bmc.1130080608

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-resolution separation of a PCR product from a mixture of DNA restriction fragments was achieved using capillary gel electrophoresis. The capillary gel electrophoretic separation gives an excellent resolution of two fragments of the 500-bp PCR product and the 506-bp DNA fragment, which differs by only 6 bp, and the complete separation of a broader chain length range of DNA afragments up to 12 kbp within 20 min. The plate number of gel-filled capillary was achieved to be 2 million plates per meter. Capillary gel electrophoresis is applied to the gene diagnosis for heart disease through apolipoprotein E genotyping. The advantages and limitations of capillary gel electrophoresis in the application to PCR analysis and DNA diagnosis are discussed.

引用

页码：291 / 293

页数：3