A METHOD FOR EVALUATING MICROSCOPE OBJECTIVES TO OPTIMIZE PERFORMANCE OF CONFOCAL SYSTEMS

被引：14

作者：

COGSWELL, CJ

SHEPPARD, CJR

MOSS, MC

HOWARD, CV

机构：

[1] UNIV OXFORD,DEPT ENGN SCI,OXFORD OX1 3PJ,ENGLAND

[2] UNIV LIVERPOOL,DEPT HUMAN ANAT & CELL BIOL,LIVERPOOL L69 3BX,ENGLAND

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1990年 / 158卷

关键词：

aberrations; Confocal microscopy; microscope objective; optical microscopy; resolution; scanning microscopy;

D O I：

10.1111/j.1365-2818.1990.tb02991.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A method for evaluating the performance of microscope objectives on two types of confocal scanning optical microscope is presented. Of these two confocal microscope types, off‐axis beam‐scanning systems are found to require microscope objectives which have been corrected for flatness of field as well as for spherical aberration and astigmatism in order to obtain maximum axial and laterial resolution. In the case of on‐axis specimen‐scanning microscopes, less highly corrected objective lenses (not corrected for flatness of field) may in practice prove to have superior resolving properties. 1990 Blackwell Science Ltd

引用

页码：177 / 185

页数：9

共 7 条

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[J]. APPLIED OPTICS, 1988, 27 (22): : 4782 - 4786
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