学术探索
学术期刊
新闻热点
数据分析
智能评审

CLEAVAGE OF BACILLUS-SUBTILIS ENDO-BETA-1,4-GLUCANASE BY B-MEGATERIUM PROTEASE

被引：9
作者：
AHN, DH
KIM, H
PACK, MY
机构：
[1] SUNCHON NATL UNIV,DEPT AGR CHEM,SUNCHON 540742,SOUTH KOREA
[2] KAIST,DEPT BIOTECHNOL,TAEJON 305338,SOUTH KOREA
来源：
BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 02期
关键词：
D O I：
10.1007/BF00133011
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A protease secreted by B. megaterium ATCC 14945 was purified by ammonium sulfate fractionation, Q-Sepharose, Sephadex G-75, and hydroxyapatite chromatography and its molecular weight was estimated to be 38 kD. The purified protease caused the cleavage of 52 kD B. subtilis endo-beta-1,4-glucanase expressed in B. megaterium. The enzyme was most active at pH 7.5 and 55-degrees-C. Calcium ion was required for the enzyme activity and the activity was almost completely inhibited by EDTA. The protease was not inhibited by phenylmethylsulfonyl fluoride.
引用
收藏
页码：127 / 132
页数：6
相关论文
共 21 条
[1]   DIFFERENCES IN THE REGULATION OF EXOCELLULAR PROTEINASE SYNTHESIS DURING GROWTH AND SPOROGENESIS OF BACILLUS-MEGATERIUM [J].
CHALOUPKA, J ;
SEVERIN, AI ;
SASTRY, KJ ;
KUCEROVA, H ;
STRNADOVA, M .
CANADIAN JOURNAL OF MICROBIOLOGY, 1982, 28 (11) :1214-1218
[2]   REPLICATION AND EXPRESSION OF PLASMIDS FROM STAPHYLOCOCCUS-AUREUS IN BACILLUS-SUBTILIS [J].
EHRLICH, SD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (04) :1680-1682
[3]  
GILKES NR, 1989, J BIOL CHEM, V264, P17802
[4]  
GILKES NR, 1988, J BIOL CHEM, V263, P10401
[5]  
KEAY L, 1971, BIOCHIM BIOPHYS ACTA, V299, P829
[6]   C-TERMINAL PROCESSING OF BACILLUS-SUBTILIS BSE616 ENDO-BETA-1,4-GLUCANASE IN BACILLUS-MEGATERIUM [J].
KIM, H ;
KIM, SMF ;
PACK, MY .
BIOTECHNOLOGY LETTERS, 1991, 13 (11) :799-804
[7]   ENDO-BETA-1,4-GLUCANASE ENCODED BY BACILLUS-SUBTILIS GENE CLONED IN BACILLUS-MEGATERIUM [J].
KIM, H ;
PACK, MY .
ENZYME AND MICROBIAL TECHNOLOGY, 1988, 10 (06) :347-351
[8]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[9]   USE OF BACILLI FOR OVERPRODUCTION OF EXOCELLULAR ENDO-BETA-1,4-GLUCANASE ENCODED BY CLONED GENE [J].
LEE, DS ;
PACK, MY .
ENZYME AND MICROBIAL TECHNOLOGY, 1987, 9 (10) :594-597
[10]   BACILLUS-SUBTILIS BETA-1,4-ENDOGLUCANASE PRODUCTS FROM INTACT AND TRUNCATED GENES ARE SECRETED INTO THE EXTRACELLULAR MEDIUM BY ESCHERICHIA-COLI [J].
LO, AC ;
MACKAY, RM ;
SELIGY, VL ;
WILLICK, GE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (09) :2287-2292
123