REQUIREMENT FOR INTRON-ENCODED U22 SMALL NUCLEOLAR RNA IN 18S RIBOSOMAL-RNA MATURATION

被引：137

作者：

TYCOWSKI, KT ^{[1
]}

SHU, MD ^{[1
]}

STEITZ, JA ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,HOWARD HUGHES MED INST,DEPT MOLEC BIOPHYS & BIOCHEM,NEW HAVEN,CT 06536

来源：

SCIENCE | 1994年 / 266卷 / 5190期

关键词：

D O I：

10.1126/science.7985025

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The nucleoli of vertebrate cells contain a number of small RNAs that are generated by the processing of intron fragments of protein-coding gene transcripts. The host gene (UHG) for intron-encoded human U22 is unusual in that it specifies a polyadenylated but apparently noncoding RNA. Depletion of U22 from Xenopus oocytes by oligonucleotide-directed ribonuclease H targeting prevented the processing of 18S ribosomal RNA (rRNA) at both ends. The appearance of 18S rRNA was restored by injection of in vitro-synthesized U22 RNA. These results identify a cellular function for an intronencoded small RNA.

引用

页码：1558 / 1561

页数：4

共 41 条

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