MULTIPLE-DROP-ARRAY (MDA) TECHNIQUE FOR THE LARGE-SCALE TESTING OF CULTURE MEDIA VARIATIONS IN HANGING MICRODROP CULTURES OF SINGLE CELL SYSTEMS .2. DETERMINATION OF PHYTOHORMONE COMBINATIONS FOR OPTIMAL DIVISION RESPONSE IN NICOTIANA-TABACUM PROTOPLAST CULTURES

被引：13

作者：

HARMS, CT

LORZ, H

POTRYKUS, I

机构：

[1] Friedrich Miescher Institute, CH-4002 Basel

来源：

PLANT SCIENCE LETTERS | 1979年 / 14卷 / 03期

关键词：

D O I：

10.1016/0304-4211(79)90076-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The effects on cell division of 7 auxin-like growth substances and 4 cytokinins were examined in Nicotiana tabacum protoplast cultures by the use of the multiple-drop-array (MDA) technique. The phytohormones were tested in 1 auxin/1 cytokinin combinations over a wide range of concentrations. Conditions were found which resulted in routine division frequencies of 90-95% for mesophyll protoplasts of wild-type and two light-sensitive mutants of tobacco. © 1979.

引用

页码：237 / 244

页数：8

共 9 条

[1] OBSERVATIONS ON MORPHOLOGY, ULTRASTRUCTURE AND REGENERATION OF TOBACCO LEAF EPIDERMAL PROTOPLASTS
DAVEY, MR
FREARSON, EM
WITHERS, LA
POWER, JB
[J]. PLANT SCIENCE LETTERS, 1974, 2 (01): : 23 - 27
[2] HARMS CT, 1978, THEOR APPL GENET, V53, P57, DOI [10.1007/BF00274331, 10.1007/BF00817833]
[3] HARMS CT, 1978, THEOR APPL GENET, V53, P49, DOI [10.1007/BF00817832, 10.1007/BF00274330]
[4] SOMATIC EMBRYOGENESIS FROM TOBACCO PROTOPLASTS
LORZ, H
POTRYKUS, I
THOMAS, E
[J]. NATURWISSENSCHAFTEN, 1977, 64 (08) : 439 - 440
[5] STREPTOMYCIN-RESISTANT PLANTS FROM CALLUS CULTURE OF HAPLOID TOBACCO
MALIGA, P
SZBREZNO.A
MARTON, L
[J]. NATURE-NEW BIOLOGY, 1973, 244 (131): : 29 - 30
[6] SOMATIC HYBRIDIZATION OF PLANTS BY FUSION OF PROTOPLASTS .1. SELECTION OF LIGHT RESISTANT HYBRIDS OF HAPLOID LIGHT SENSITIVE VARIETIES OF TOBACCO
MELCHERS, G
LABIB, G
[J]. MOLECULAR AND GENERAL GENETICS, 1974, 135 (04): : 277 - 294
[7] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES
MURASHIGE, T
SKOOG, F
[J]. PHYSIOLOGIA PLANTARUM, 1962, 15 (03) : 473 - 497
[8] PLATING OF ISOLATED TOBACCO MESOPHYLL PROTOPLASTS ON AGAR MEDIUM
NAGATA, T
TAKEBE, I
[J]. PLANTA, 1971, 99 (01) : 12 - +
[9] MULTIPLE-DROP-ARRAY (MDA) TECHNIQUE FOR THE LARGE-SCALE TESTING OF CULTURE MEDIA VARIATIONS IN HANGING MICRODROP CULTURES OF SINGLE CELL SYSTEMS .1. TECHNIQUE
POTRYKUS, I
HARMS, CT
LORZ, H
[J]. PLANT SCIENCE LETTERS, 1979, 14 (03): : 231 - 235

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