MULTIPLE-DROP-ARRAY (MDA) TECHNIQUE FOR THE LARGE-SCALE TESTING OF CULTURE MEDIA VARIATIONS IN HANGING MICRODROP CULTURES OF SINGLE CELL SYSTEMS .1. TECHNIQUE

被引：19

作者：

POTRYKUS, I

HARMS, CT

LORZ, H

机构：

[1] Friedrich Miescher Institute, CH-4002 Basel

来源：

PLANT SCIENCE LETTERS | 1979年 / 14卷 / 03期

关键词：

D O I：

10.1016/0304-4211(79)90075-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A novel approach to the rapid testing of numerous combinations of culture media components is presented. The 'multiple-drop-array' (MDA) technique uses 40 μl hanging drops as its experimental unit, arranged in a geometrical order according to the combination of factor gradients. It is suited for all single cell systems, including protoplasts, cells and microspores. The technique allows a small team to test 5000 media variations in 2- and 3-factor combinations with a single population of 2 × 106 cells within two days. © 1979.

引用

页码：231 / 235

页数：5

共 5 条

[1] PROTOPLAST TECHNOLOGY IN RELATION TO CROP PLANTS - PROGRESS AND PROBLEMS
BHOJWANI, SS
EVANS, PK
COCKING, EC
[J]. EUPHYTICA, 1977, 26 (02) : 343 - 360
[2] COCKING EC, 1976, SEMAINE ETUDE CEREAL, P87
[3] MULTIPLE-DROP-ARRAY (MDA) TECHNIQUE FOR THE LARGE-SCALE TESTING OF CULTURE MEDIA VARIATIONS IN HANGING MICRODROP CULTURES OF SINGLE CELL SYSTEMS .2. DETERMINATION OF PHYTOHORMONE COMBINATIONS FOR OPTIMAL DIVISION RESPONSE IN NICOTIANA-TABACUM PROTOPLAST CULTURES
HARMS, CT
LORZ, H
POTRYKUS, I
[J]. PLANT SCIENCE LETTERS, 1979, 14 (03): : 237 - 244
[4] CALLUS FORMATION FROM STEM PROTOPLASTS OF CORN (ZEA-MAYS-L)
POTRYKUS, I
HARMS, CT
LORZ, H
THOMAS, E
[J]. MOLECULAR & GENERAL GENETICS, 1977, 156 (03): : 347 - 350
[5] POTRYKUS I, THEOR APPL GENET

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