RARE SCLERODERMA AUTOANTIBODIES TO THE U11 SMALL NUCLEAR RIBONUCLEOPROTEIN AND TO THE TRIMETHYLGUANOSINE CAP OF U-RNA SMALL NUCLEAR-RNA

被引:19
作者
GILLIAM, AC
STEITZ, JA
机构
[1] YALE UNIV,SCH MED,DEPT DERMATOL,NEW HAVEN,CT 06510
[2] YALE UNIV,SCH MED,DEPT MOLEC BIOCHEM & BIOPHYS,NEW HAVEN,CT 06510
[3] HOWARD HUGHES MED INST,NEW HAVEN,CT 06510
关键词
D O I
10.1073/pnas.90.14.6781
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have identified a scleroderma serum (Ru) with a previously undescribed specificity to protein components of the U11 small nuclear ribonucleoprotein particle (snRNP), a low-abundance member of the Sm class of U RNPs. The U11 RNP can be specifically immunoprecipitated from sonicated HeLa cells with Ru serum. In nuclear extracts, a fraction of the U11 particle is found complexed to the U12 RNP, an even lower abundance Sm snRNP. In glycerol gradient fractions, Ru serum identifies a 65-kDa protein that cosediments with the U11-U12 complex and is shifted upon targeted degradation of the U12 RNA. The 65-kDa protein therefore appears to be a component of the U11-U12 snRNP complex, whereas another Ru-reactive (140 kDa) protein may be associated with the free U11 RNP. The Ru serum also contains autoantibodies directed against the trimethylguanosine cap of U RNAs. This rare specificity has been described previously in only three other scleroderma patients.
引用
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页码:6781 / 6785
页数:5
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