HYDROLYSIS OF MICROCRYSTALLINE CELLULOSE BY CELLULOLYTIC COMPLEX OF TRICHODERMA-REESEI IN LOW-MOISTURE MEDIA

被引:8
作者
SINEIRO, J [1 ]
DOMINGUEZ, H [1 ]
NUNEZ, MJ [1 ]
LEMA, JM [1 ]
机构
[1] UNIV SANTIAGO DE COMPOSTELA, DEPT CHEM ENGN, E-15706 SANTIAGO, SPAIN
关键词
TRICHODERMA REESEI; CELLULOSE; CELLULASE; LOW MOISTURE; THERMAL STABILITY;
D O I
10.1016/0141-0229(94)00107-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The activity of the extracellular cellulolytic complex of Trichoderma reesei upon crystalline cellulose was studied in the range of 4-10% moisture (dry basis) and 20-90 degrees C temperature with the aid of a central composite experimental design. An empirical model was established for both the endoglucanase and synergistic endo- and exoglucanase activities with a significance level >95%. The thermal stability of the cellulolytic complex adsorbed onto cellulose at low moisture conditions was determined and compared with that of the enzyme in liquid form; the former was more stable.
引用
收藏
页码:809 / 815
页数:7
相关论文
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[21]  
WASSERMAN BP, 1984, FOOD TECHNOL, V18, P79