PREPARATION OF LIPID VESICLES CONTAINING HIGH-LEVELS OF ENTRAPPED RADIOACTIVE CATIONS

被引：71

作者：

MAUK, MR

GAMBLE, RC

机构：

[1] Division of Chemistry and Chemical Engineering, Arthur Amos Noves Laboratory of Chemical Physics, California Institute of Technology, Pasadena

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1016/0003-2697(79)90364-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ionophore A23187 was incorporated into the lipid bilayer of unilamellar vesicles and used to earry externally added indium ions to a chelator, nitrilotriacetic acid, which was previously entrapped in the vesicles. The extent of vesicle loading was assayed by Sephadex chromatography and γ-ray perturbed angular correlation spectroscopy. Incubation conditions were developed which allow rapid loading of more than 90% of added 121In3+ with specific activities of 200-300 μCi/mg lipid. © 1979.

引用

页码：302 / 307

页数：6