ANALYSIS OF ERYTHROMYCIN ESTOLATE BY LIQUID-CHROMATOGRAPHY

被引：18

作者：

CACHET, T ^{[1
]}

DELRUE, M ^{[1
]}

PAESEN, J ^{[1
]}

BUSSON, R ^{[1
]}

ROETS, E ^{[1
]}

HOOGMARTENS, J ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,INST FARMACEUT WETENSCHAPPEN,FARMACEUT CHEM LAB,VAN EVENSTRAAT 4,B-3000 LOUVAIN,BELGIUM

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1992年 / 10卷 / 10-12期

关键词：

ERYTHROMYCIN ESTOLATE; REVERSED-PHASE LIQUID CHROMATOGRAPHY;

D O I：

10.1016/0731-7085(91)80091-M

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described for the determination of erythromycin estolate by liquid chromatography. A C18 reversed-phase column (25 x 0.46 cm i.d.) was used with acetonitrile-tetrabutylammonium sulphate (pH 6.5, 0.2 M)-phosphate buffer (pH 6.5, 0.2 M)-water [x:5:5:(90-x), v/v/v/v] as mobile phase. The proportion of acetonitrile (x) has to be adapted to the type of stationary phase used. For RSil C18 LL 42.5% (v/v) was used. The column was heated at 35-degrees-C, the flow rate was 1.5 ml min-1 and UV detection was performed at 215 nm. The main component, erythromycin A propionate, was separated from all other components which were present in commercial samples. The impurities most frequently observed were the propionate ester of erythromycin C and the amide N-propionyl-N-demethylerythromycin A. Erythromcin A was shown to be present in specialties.

引用

页码：851 / 860

页数：10