LIGNIN PEROXIDASES ARE OLIGOMANNOSE TYPE GLYCOPROTEINS

被引：15

作者：

SCHMIDT, B ^{[1
]}

HEIMGARTNER, U ^{[1
]}

KOZULIC, B ^{[1
]}

LEISOLA, MSA ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,DEPT BIOTECHNOL,CH-8092 ZURICH,SWITZERLAND

来源：

JOURNAL OF BIOTECHNOLOGY | 1990年 / 13卷 / 2-3期

关键词：

(Phanerochaete chrysosporium); Glycoprotein structure; Lignin peroxidase;

D O I：

10.1016/0168-1656(90)90107-M

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The glycoprotein nature of five lignin peroxidases has been investigated. By gas-liquid chromatography the isoenzymes were found to contain two N-acetylglucosamines and from 14 to 18 mannose residues. Deglycosylation by Endo H decreased the apparent molecular weight of all isoenzymes by about 2 kDa. These results suggest that lignin peroxidases contain one N-linked sugar chain. In addition, O-linked monosaccharides are also present because the deglycosylated isoenzymes stained strongly with a glycoprotein specific staining and treatment with a strong base resulted in the characteristic increase of absorbance at 241 nm. © 1990.

引用

页码：223 / 228

页数：6

共 19 条

[1] ANALYSIS OF PROTEIN AND PEPTIDE MIXTURES - EVALUATION OF 3 SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS BUFFER SYSTEMS [J].

BURY, AF .

JOURNAL OF CHROMATOGRAPHY, 1981, 213 (03) :491-500

[2]

CARLSON DM, 1968, J BIOL CHEM, V243, P616

[3] A RAPID AND SENSITIVE METHOD FOR THE ANALYSIS OF CARBOHYDRATE COMPONENTS IN GLYCOPROTEINS USING GAS-LIQUID-CHROMATOGRAPHY [J].

CHAPLIN, MF .

ANALYTICAL BIOCHEMISTRY, 1982, 123 (02) :336-341

[4] AN EXTRACELLULAR H2O2-REQUIRING ENZYME PREPARATION INVOLVED IN LIGNIN BIODEGRADATION BY THE WHITE ROT BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM [J].

GLENN, JK ;

MORGAN, MA ;

MAYFIELD, MB ;

KUWAHARA, M ;

GOLD, MH .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 114 (03) :1077-1083

[5] MONOCLINIC CRYSTALS OF LIGNIN PEROXIDASE [J].

GLUMOFF, T ;

WINTERHALTER, KH ;

SMIT, JDG .

FEBS LETTERS, 1989, 257 (01) :59-62

[6]

HAEMMERLI S, 1986, FEMS MICROBIOL LETT, V53, P33

[7] POLYACRYLIC POLYHYDRAZIDES AS REAGENTS FOR DETECTION OF GLYCOPROTEINS [J].

HEIMGARTNER, U ;

KOZULIC, B ;

MOSBACH, K .

ANALYTICAL BIOCHEMISTRY, 1989, 181 (01) :182-189

[8]

Johnson D. A., 1984, Gene Analysis Techniques, V1, P3, DOI 10.1016/0735-0651(84)90049-9

[9] ENZYMATIC COMBUSTION - THE MICROBIAL-DEGRADATION OF LIGNIN [J].

KIRK, TK ;

FARRELL, RL .

ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :465-505

[10] N-ACETYLATION OF AMINO SUGAR METHYL GLYCOSIDES FOR GAS-LIQUID-CHROMATOGRAPHIC ANALYSIS [J].

KOZULIC, B ;

RIES, B ;

MILDNER, P .

ANALYTICAL BIOCHEMISTRY, 1979, 94 (01) :36-39

← 1 2 →