DETECTION OF FETAL RHD SEQUENCE FROM PERIPHERAL-BLOOD OF SENSITIZED RHD-NEGATIVE PREGNANT-WOMEN

被引：22

作者：

LO, YMD

NOAKES, L

BOWELL, PJ

FLEMING, KA

WAINSCOAT, JS

机构：

[1] JOHN RADCLIFFE HOSP,DEPT HAEMATOL,OXFORD OX3 9DU,ENGLAND

[2] JOHN RADCLIFFE HOSP,NUFFIELD DEPT CLIN MED,OXFORD OX3 9DU,ENGLAND

[3] JOHN RADCLIFFE HOSP,REG TRANSFUS LAB,OXFORD,ENGLAND

[4] JOHN RADCLIFFE HOSP,NUFFIELD DEPT PATHOL & BACTERIOL,OXFORD OX3 9DU,ENGLAND

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1994年 / 87卷 / 03期

基金：

英国惠康基金;

关键词：

RHESUS HEMOLYTIC DISEASE; FETAL CELLS IN MATERNAL BLOOD; PCR; NONINVASIVE PRENATAL DIAGNOSIS; BLOOD GROUPS;

D O I：

10.1111/j.1365-2141.1994.tb08336.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A sensitive PCR-based assay was developed to amplify fetal-derived rhesus D (RhD) sequence from peripheral blood of RhD-negative pregnant women with circulating anti-D. RhD-PCR positivity was detected in 7/22 samples from women bearing RhD-positive fetuses, despite the presence of varying levels of anti-D. Evidence is presented which suggests that rising maternal anti-D levels might reduce circulating fetal cell numbers. Further development of this assay may have implications in the clinical management of RhD-sensitized pregnancies and aid the understanding of the physiology of feto-maternal cell trafficking.

引用

页码：658 / 660

页数：3

共 11 条

[1] ISOLATION OF FETAL DNA FROM NUCLEATED ERYTHROCYTES IN MATERNAL BLOOD [J].