ENZYMATIC ASSAYS BASED ON THE COULOMETRIC MICROFLOW TITRATION OF AMMONIA AND CARBON-DIOXIDE

Rapidly and precisely working microflow titrations are suitable to follow enzyme catalyzed reactions producing ammonia and carbon dioxide. Ammonia and carbon dioxide were separated from the reaction zone by a gas dialysis step. Highly precise and accurate determinations of urea. glutamine, glutamate, asparagine, carbon dioxide and ammonia can be implemented. The enzymes are immobilized covalently on a preactivated membrane sandwiched with a microporous Teflon membrane. A compact and fully automated microcoulometric measuring system for fast and long term stable enzymatic determinations was developed.