PHYSICAL ORDERING OF 3 POLYMORPHIC DNA MARKERS SPANNING THE REGIONS CONTAINING A TUMOR SUPPRESSOR GENE OF RENAL-CELL CARCINOMA BY 3-COLOR FLUORESCENT INSITU HYBRIDIZATION

被引：50

作者：

INAZAWA, J

ARIYAMA, T

ABE, T

机构：

[1] Department of Hygiene, Kyoto Prefectural University of Medicine, Kyoto, 602, Kajii‐cho, Hirokoji‐Kawaramachi, Kamigyo‐ku

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1992年 / 83卷 / 12期

关键词：

3-COLOR FISH; PHYSICAL ORDERING; RFLP MARKER; RENAL CELL CARCINOMA;

D O I：

10.1111/j.1349-7006.1992.tb02753.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Fluorescent in situ hybridization (FISH) is a powerful technique for gene mapping and multi-color FISH allows us to determine directly the order of two or more probes on both metaphase and interphase chromosomes. We report the physical ordering of three DNA markers by three-color FISH using two fluorochrome dyes, fluorescein isothiocyanate (FITC; green) and rhodamine (red). The third color was visualized as a pseudo-color (yellow) generated by optical interference with FITC and rhodamine. Using this system we could rapidly determine the order of three polymorphic DNA markers located on the 3p23-p21.2 bands which span a tumor suppressor gene for renal cell carcinoma, lung carcinoma, and several other types of tumors.

引用

页码：1248 / 1252

页数：5

共 30 条

[1]

BERGERHEIM U, 1989, CANCER RES, V49, P1390

[2] A NEW SYSTEM FOR HIGH-RESOLUTION DNA-SEQUENCE MAPPING IN INTERPHASE PRONUCLEI [J].