DNA AMPLIFICATION FINGERPRINTING USING ARBITRARY MINI-HAIRPIN OLIGONUCLEOTIDE PRIMERS

被引：42

作者：

CAETANOANOLLES, G ^{[1
]}

GRESSHOFF, PM ^{[1
]}

机构：

[1] UNIV TENNESSEE, CTR LEGUME RES, KNOXVILLE, TN 37901 USA

来源：

BIO-TECHNOLOGY | 1994年 / 12卷 / 06期

关键词：

D O I：

10.1038/nbt0694-619

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The enzymatic amplification of DNA directed by very short oligonucleotides of arbitrary sequence produces complex DNA profiles useful for genome analysis and identity testing. Mini-hairpins harboring a ''core'' arbitrary sequence at the 3' terminus primed the amplification of a wide range of templates ranging from plasmid DNA to plant and animal genomes. Primer core regions of only 3 nucleotides produced complex fingerprints, but the hairpin sequence also influenced the amplification reaction. Oligonucleotide substitution with degenerate bases tailored the complexity of fingerprint patterns. Simulation studies of the amplification of plasmids pUC18 and pBR322 using primers with short arbitrary cores allowed assignment of amplification products to expected regions and revealed physical interaction between annealing sites during amplification of first-round products. Mini-hairpin primers can increase detection of polymorphic DNA, and be used to study subgenomic fragments like yeast artificial chromosomes (YACs), cloned DNA and PCR products.

引用

页码：619 / 623

页数：5

共 21 条

[1] THERMODYNAMIC PARAMETERS FOR LOOP FORMATION IN RNA AND DNA HAIRPIN TETRALOOPS [J].

ANTAO, VP ;

TINOCO, I .

NUCLEIC ACIDS RESEARCH, 1992, 20 (04) :819-824

[2] A THERMODYNAMIC STUDY OF UNUSUALLY STABLE RNA AND DNA HAIRPINS [J].

ANTAO, VP ;

LAI, SY ;

TINOCO, I .

NUCLEIC ACIDS RESEARCH, 1991, 19 (21) :5901-5905

[3] SILVER STAINING OF DNA IN POLYACRYLAMIDE GELS [J].

BASSAM, BJ ;

CAETANOANOLLES, G .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1993, 42 (2-3) :181-188

[4]

BASSAM BJ, 1992, APPL MICROBIOL BIOT, V38, P70, DOI 10.1007/BF00169422

[5] FAST AND SENSITIVE SILVER STAINING OF DNA IN POLYACRYLAMIDE GELS [J].

BASSAM, BJ ;

CAETANOANOLLES, G ;

GRESSHOFF, PM .

ANALYTICAL BIOCHEMISTRY, 1991, 196 (01) :80-83

[6] ENHANCED DETECTION OF POLYMORPHIC DNA BY MULTIPLE ARBITRARY AMPLICON PROFILING OF ENDONUCLEASE-DIGESTED DNA - IDENTIFICATION OF MARKERS TIGHTLY LINKED TO THE SUPERNODULATION LOCUS IN SOYBEAN [J].

CAETANOANOLLES, G ;

BASSAM, BJ ;

GRESSHOFF, PM .

MOLECULAR AND GENERAL GENETICS, 1993, 241 (1-2) :57-64

[7] PRIMER-TEMPLATE INTERACTIONS DURING DNA AMPLIFICATION FINGERPRINTING WITH SINGLE ARBITRARY OLIGONUCLEOTIDES [J].

CAETANOANOLLES, G ;

BASSAM, BJ ;

GRESSHOFF, PM .

MOLECULAR AND GENERAL GENETICS, 1992, 235 (2-3) :157-165

[8] DNA AMPLIFICATION FINGERPRINTING USING VERY SHORT ARBITRARY OLIGONUCLEOTIDE PRIMERS [J].

CAETANOANOLLES, G ;

BASSAM, BJ ;

GRESSHOFF, PM .

BIO-TECHNOLOGY, 1991, 9 (06) :553-557

[9]

CAETANOANOLLES G, 1993, PCR METH APPL, V3, P85

[10] DNA AMPLIFICATION FINGERPRINTING USING ARBITRARY OLIGONUCLEOTIDE PRIMERS [J].

CAETANOANOLLES, G ;

BASSAM, BJ .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1993, 42 (2-3) :189-200

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