THE T-CELL REACTIVITY AGAINST THE MAJOR MEROZOITE PROTEIN OF PLASMODIUM-FALCIPARUM

被引：9

作者：

CRISANTI, A ^{[1
]}

FRUH, K ^{[1
]}

MULLER, HM ^{[1
]}

BUJARD, H ^{[1
]}

机构：

[1] UNIV ROME LA SAPIENZA,IST PARASSITOL,I-00185 ROME,ITALY

来源：

IMMUNOLOGY LETTERS | 1990年 / 25卷 / 1-3期

关键词：

Dimorphic sequence; Plasmodium falciparum gp190; T cell epitopes;

D O I：

10.1016/0165-2478(90)90106-Z

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have undertaken a systematic search for T cell epitopes within the sequence of the major merozoite surface antigen (GP190) of Plasmodium falciparum. Recombinant polypeptides expressed in E. coli were used to evaluate the reactivity of peripheral blood monuclear cells (PBMC) from both inhabitants of a rural community of West Africa exposed to P. falciparum transmission and from German patients with diagnosis of acute malaria. Although the proliferative response of the PBMC was in most cases very low, several T cell clones could be established. Deletion analysis of each gp190-derived polypeptide allowed the identification of six different T cell epitopes. Epitopes could be mapped within the dimorphic region of gp190, which also contains the sequences most frequently recognized by sera from adult individuals living in endemic areas. © 1990.

引用

页码：143 / 148

页数：6

共 19 条

[1] MONOCLONAL-ANTIBODIES THAT PROTECT INVIVO AGAINST PLASMODIUM-CHABAUDI RECOGNIZE A 250,000-DALTON PARASITE POLYPEPTIDE [J].

BOYLE, DB ;

NEWBOLD, CI ;

SMITH, CC ;

BROWN, KN .

INFECTION AND IMMUNITY, 1982, 38 (01) :94-102

[2] EPITOPES RECOGNIZED BY HUMAN T-CELLS MAP WITHIN THE CONSERVED PART OF THE GP190 OF P-FALCIPARUM [J].

CRISANTI, A ;

MULLER, HM ;

HILBICH, C ;

SINIGAGLIA, F ;

MATILE, H ;

MCKAY, M ;

SCAIFE, J ;

BEYREUTHER, K ;

BUJARD, H .

SCIENCE, 1988, 240 (4857) :1324-1326

[3] T-CELL ANTIGENIC SITES TEND TO BE AMPHIPATHIC STRUCTURES [J].

DELISI, C ;

BERZOFSKY, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (20) :7048-7052

[4]

EPSTEIN N, 1981, J IMMUNOL, V127, P212

[5]

FRUH K, 1990, UNPUB

[6] MAJOR SURFACE-ANTIGEN GENE OF A HUMAN MALARIA PARASITE CLONED AND EXPRESSED IN BACTERIA [J].

HALL, R ;

HYDE, JE ;

GOMAN, M ;

SIMMONS, DL ;

HOPE, IA ;

MACKAY, M ;

SCAIFE, J ;

MERKLI, B ;

RICHLE, R ;

STOCKER, J .

NATURE, 1984, 311 (5984) :379-382

[7] ANTIGEN-SPECIFIC IMMUNOSUPPRESSION IN HUMAN MALARIA DUE TO PLASMODIUM-FALCIPARUM [J].

HO, M ;

WEBSTER, HK ;

LOOAREESUWAN, S ;

SUPANARANOND, W ;

PHILLIPS, RE ;

CHANTHAVANICH, P ;

WARRELL, DA .

JOURNAL OF INFECTIOUS DISEASES, 1986, 153 (04) :763-771

[8] GENETIC APPROACH TO FACILITATE PURIFICATION OF RECOMBINANT PROTEINS WITH A NOVEL METAL CHELATE ADSORBENT [J].

HOCHULI, E ;

BANNWARTH, W ;

DOBELI, H ;

GENTZ, R ;

STUBER, D .

BIO-TECHNOLOGY, 1988, 6 (11) :1321-1325

[9] THE 3 MAJOR ANTIGENS ON THE SURFACE OF PLASMODIUM-FALCIPARUM MEROZOITES ARE DERIVED FROM A SINGLE HIGH MOLECULAR-WEIGHT PRECURSOR [J].

HOLDER, AA ;

FREEMAN, RR .

JOURNAL OF EXPERIMENTAL MEDICINE, 1984, 160 (02) :624-629

[10] Immunization against blood-stage rodent malaria using purified parasite antigens [J].

Holder, Anthony A. ;

Freeman, Robert R. .

NATURE, 1981, 294 (5839) :361-364

← 1 2 →