DETERMINATION OF ERYTHROCYTE AMINO-ACIDS BY GAS-CHROMATOGRAPHY

被引：9

作者：

LEIGHTON, WP ^{[1
]}

ROSENBLATT, S ^{[1
]}

CHANLEY, JD ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED,DEPT BIOCHEM,NEW YORK,NY 10029

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 164卷 / 04期

关键词：

D O I：

10.1016/S0378-4347(00)81546-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Erythrocyte amino acid levels were determined, by gas chromatography, in a group of 34 normal human adults. No significant sex or age correlations were noted. A method for the quantitative gas chromatographic analysis of free amino acids in erythrocytes is described. Following hemolysis and deproteinization the amino acids were isolated on a cation-exchange resin. Glutathione was removed from the amino acid mixture by adsorption on an anion-exchange resin. Following conversion to their N-acetyl-n-propyl esters, 19 amino acids were separated and quantitated by gas chromatography on a single column in 18 min. Typical reproducibility data indicate that a coefficient of variation of 2-5% is attainable. © 1979.

引用

页码：427 / 439

页数：13

共 40 条

[1] DETERMINATION OF AMINO-ACID PROFILES IN BIOLOGICAL SAMPLES BY GAS-CHROMATOGRAPHY [J].