DRASTIC G2 ARREST IN MAMMALIAN-CELLS AFTER IRRADIATION WITH HEAVY-ION BEAMS

被引：103

作者：

LUCKEHUHLE, C ^{[1
]}

BLAKELY, EA ^{[1
]}

CHANG, PY ^{[1
]}

TOBIAS, CA ^{[1
]}

机构：

[1] KERNFORSCHUNGSZENTRUM KARLSRUHE,INST GENET & TOKIKOL,D-7500 KARLSRUHE,FED REP GER

来源：

RADIATION RESEARCH | 1979年 / 79卷 / 01期

关键词：

D O I：

10.2307/3575025

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Unsynchronized exponentially growing V79 monolayer cultures were exposed to high-energy radiation heavy-ion beams (12C, 20Ne, 40Ar) at various positions in their unmodified Bragg ionization curves. High-LET particle beams proved to be much more effective in causing a G2 + M block, measured by the method of flow microfluorometry, than low-LET radiation. The number of cells arrested in G2 + M increased linearly with dose up to at least 75% of the maximum effect. In this domain, the RBE values were LET-dependent with a maximum (RBE50 = 8.3) at about 100 keV/μm. In contrast to γ and X rays, heavy ions with high LET did not affect cell traverse through the G1 and S phase. Though the RBE values for G2 + M block are much higher than the RBEs for survival, there was a linear relationship between accumulation of cells in G2 + M and cell killing.

引用

页码：97 / 112

页数：16