FUNCTIONAL COMPLEMENTATION IN MOUSE-HUMAN RADIATION HYBRIDS ASSIGNS THE PUTATIVE MURINE SCID GENE TO THE PERICENTRIC REGION OF HUMAN CHROMOSOME-8

被引：45

作者：

KOMATSU, K ^{[1
]}

OHTA, T ^{[1
]}

JINNO, Y ^{[1
]}

NIIKAWA, N ^{[1
]}

OKUMURA, Y ^{[1
]}

机构：

[1] NAGASAKI UNIV,SCH MED,INST ATOM DIS,DEPT HUMAN GENET,NAGASAKI 852,JAPAN

来源：

HUMAN MOLECULAR GENETICS | 1993年 / 2卷 / 07期

关键词：

D O I：

10.1093/hmg/2.7.1031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fragments of human chromosome 8 were introduced into cells derived from murine scid mice via X-irradiation and somatic cell fusion. The resulting hybrid clones contained human DNA fragment(s) which complemented the hyper-radiosensitivity of the scid cells. Alu-PCR products from these hybrids were used for chromosome painting using the technique of chromosome in situ suppression hybridization, allowing assignment of the human HYRC (hyper-radiosensitivity of murine scid mutation, complementing) gene, a candidate for a V(D)J recombinase gene, to human chromosome 8q11.

引用

页码：1031 / 1034

页数：4

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