DNA TYPING WITH DIGOXIGENIN-11-DIDEOXYURIDINETRIPHOSPHATE-LABELED OLIGONUCLEOTIDE PROBES ENABLES NONRADIOACTIVE ANALYSIS USING A DUAL DETECTION SYSTEM - APPLICATION FOR SCREENING HLA-DQA1 POLYMORPHISMS

被引：7

作者：

RIHS, HP ^{[1
]}

THIELE, A ^{[1
]}

PERICHON, B ^{[1
]}

KRISHNAMOORTHY, R ^{[1
]}

BAUR, X ^{[1
]}

机构：

[1] HOP ROBERT DEBRE, INSERM, U120, PARIS, FRANCE

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 1993年 / 101卷 / 01期

关键词：

3-(2'-SPIROADAMANTAN)-4-(3''-PHOSPHORYLOXY)PHENYL-1,2-DIOXYETHANE; DIGOXIGENIN; DQA1; OLIGONUCLEOTIDES; SEQUENCE-SPECIFIC;

D O I：

10.1159/000236492

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

We describe a standardized, highly sensitive, nonradioactive detection procedure for HLA class-II typing of DQA1 alleles and suballeles which has important and cost-effective application in studying HLA disease associations. The procedure involves polymerase chain reaction-based target DNA sequence amplification and dot blotting followed by stringent hybridization with digoxigenin-11-dideoxyuridine triphosphate 3'-end-labelled allele-specific oligonucleotide probes. The dual detection system described here makes this procedure very versatile.

引用

收藏

页码：7 / 12

页数：6

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