RECOMBINANT CDNA ENCAPSULATION IN SMALL LIPOSOMES WITH HEPATOCYTE ACCESS ABILITY

被引:9
作者
ALINO, SF
BOBADILLA, M
UNDA, FJ
GARCIASANZ, M
HILARIO, E
LEJARRETA, M
机构
[1] Department of Pharmacology, Faculty of Medicine and Density, University of Valencia, 46010, Valencia
[2] Department of Cell Biology and Morphological Sciences, Faculty of Medicine and Dentistry, University of the Basque Country, Leioa, 48940, Vizcaya
关键词
D O I
10.3109/02652049309104382
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Liposomal encapsulation efficiency of a recombinant cDNA was studied by several procedures. We observed that supernatant fraction of ultracentrifuged liposomes prepared by extrusion through polycarbonate filters of 400 nm pore size yielded a very homogeneous suspension of small (50 nm diameter) unilamellar liposomes with highest DNA/lipid ratio and great ability to access to hepatocytes.
引用
收藏
页码:163 / 170
页数:8
相关论文
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