CLONING OF THE RAT ANGIOTENSIN-II TYPE-2 RECEPTOR GENE AND IDENTIFICATION OF ITS FUNCTIONAL PROMOTER REGION

被引：21

作者：

KOBAYASHI, S

OHNISHI, J

NIBU, Y

NISHIMATSU, S

UMEMURA, S

ISHII, M

MURAKAMI, K

MIYAZAKI, H

机构：

[1] UNIV TSUKUBA,CTR GENE EXPT,INST APPL BIOCHEM,TSUKUBA,IBARAKI 305,JAPAN

[2] YOKOHAMA CITY UNIV,SCH MED,DEPT INTERNAL MED 2,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1262卷 / 2-3期

关键词：

ANGIOTENSIN II; TYPE; 2; RECEPTOR; CHLORAMPHENICOL ACETYLTRANSFERASE;

D O I：

10.1016/0167-4781(95)00076-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned the rat angiotensin II receptor type 2 (AT(2)) gene, whose physiological function remains unclear. Sequence analysis indicated that exons 1 and 2 exist in the 5'-untranslated region and the initiation codon ATG is located in exon 3. The 1.6-kb genomic fragment at positions -1567 to +26 relative to the putative transcription start site was found to contain a functional promoter region using transient chloramphenicol acetyltransferase assay. This is the first report demonstrating the nucleotide sequence of the promoter region of this gene.

引用

页码：155 / 158

页数：4