CYTOSININE - PYRIDOXAL-PHOSPHATE TAUTOMERASE, A NEW ENZYME IN THE BLASTICIDIN-S BIOSYNTHETIC-PATHWAY

被引：10

作者：

GOULD, SJ

ZHANG, QB

机构：

[1] Department of Chemistry, Oregon State University

来源：

JOURNAL OF ANTIBIOTICS | 1995年 / 48卷 / 07期

关键词：

D O I：

10.7164/antibiotics.48.652

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cytosinine-the nucleoside portion of blasticidin S-and pyridoxal phosphate were incubated with cell-free extracts of Streptomyces griseochromogenes prepared in D2O. H-2 NMR analysis of recovered cytosinine showed it to contain deuterium enrichments at H-4' and H-2' No exchange was observed with either boiled extract or from cytosinine and pyridoxal phosphate alone. These results reveal the presence of a tautomerase activity that contributes to the net transamination at C-4' in the conversion of cytosylglucuronic acid to blasticidin S, and its discovery supports the role of cytosinine as a biosynthetic intermediate.

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页码：652 / 656

页数：5

相关论文

共 26 条

[1] USE OF FUNGAL PROTOPLASTS IN STUDY OF AFLATOXIN BIOSYNTHESIS [J].

DUTTON, MF ;

ANDERSON, MS .

EXPERIENTIA, 1978, 34 (01) :22-24

[2]

FOX JJ, 1966, TETRAHEDRON LETT, P897

[3]

GAVREAU D, 1984, CAN J MICROBIOL, V30, P721

[4] STEREOCHEMISTRY OF C-3 DEOXYGENATION OF SUGAR NUCLEOSIDES - FORMATION OF PENTOPYRANINE-C FROM [3-H-2]-D-GLUCOSE BY STREPTOMYCES-GRISEOCHROMOGENES [J].

GOULD, SJ ;

GUO, JC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (26) :10176-10181

[5] NUCLEOSIDE INTERMEDIATES IN BLASTICIDIN-S BIOSYNTHESIS IDENTIFIED BY THE IN-VIVO USE OF ENZYME-INHIBITORS [J].

GOULD, SJ ;

GUO, JC ;

GEITMANN, A ;

DEJESUS, K .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1994, 72 (01) :6-11

[6] CYTOSYLGLUCURONIC ACID SYNTHASE (CYTOSINE-UDP-GLUCURONOSYLTRANSFERASE) FROM STREPTOMYCES GRISEOCHROMOGENES, THE FIRST PROKARYOTIC UDP-GLUCURONOSYLTRANSFERASE [J].

GOULD, SJ ;

GUO, JC .

JOURNAL OF BACTERIOLOGY, 1994, 176 (05) :1282-1286

[7] BIOSYNTHESIS OF BLASTICIDIN-S FROM CYTOSYLGLUCURONIC ACID (CGA) - ISOLATION OF CYTOSINE UDPGLUCURONOSYLTRANSFERASE AND INCORPORATION OF CGA BY STREPTOMYCES-GRISEOCHROMOGENES [J].

GUO, JC ;

GOULD, SJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (15) :5898-5899

[8] BIOSYNTHESIS OF BLASTICIDIN-S - CELL-FREE DEMONSTRATION OF N-METHYLATION AS THE LAST STEP [J].

GUO, JC ;

GOULD, SJ .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1991, 1 (10) :497-500

[9] CYTOSINE GLYCOSIDES FROM STREPTOMYCES-GRISEOCHROMOGENES [J].

GUO, JC ;

GOULD, SJ .

PHYTOCHEMISTRY, 1993, 32 (03) :535-541

[10]

HAN O, 1990, J BIOL CHEM, V265, P8033