HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF CAFFEINE, THEOPHYLLINE, THEOBROMINE AND PARAXANTHINE IN RAT-BRAIN AND SERUM

被引：17

作者：

PARRA, P ^{[1
]}

LIMON, A ^{[1
]}

FERRE, S ^{[1
]}

GUIX, T ^{[1
]}

JANE, F ^{[1
]}

机构：

[1] HOSP SANT PAU, PROGRAMA SANT PAU CITRAN, FDN INVEST SANT PAU, NEUROPSICOFARMACOL LAB, BARCELONA, SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 570卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80214-W

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Caffeine and its metabolites theophylline, theobromine and paraxanthine have been determined in rat brain and serum samples by high-performance liquid chromatography with ultraviolet detection. The recovery, 85-103%, allowed quantification by external standard methods. The variability was found to be less than 3 and 7% for intra-day and inter-day assays, respectively. The detection limit, 1.57 ng of methylxanthines on column, allowed the determination of 62.5 ng/g or ml in biological material. Rats treated with 30 mg/kg caffeine (subcutaneously) were sacrificed at different times (1, 6, 12 and 24 h). Higher concentrations of methylxanthines (specially paraxanthine) were observed in the striatum than in the rest of the brain, and it was also observed that the clearance of methylxanthines was faster in serum than in brain structures.

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页码：185 / 190

页数：6

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