共 21 条
[21]
WILL OH, 1984, LC LIQ CHROMATOGR HP, V2, P610
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF THE GEOMETRICAL-ISOMERS OF BETA-CAROTENE IN SEVERAL FOODSTUFFS
被引：6
作者：
CARVALHO, CRL
CARVALHO, PRN
COLLINS, CH
机构：
[1] UNIV ESTADUAL CAMPINAS, INST QUIM, BR-13083970 CAMPINAS, BRAZIL
[2] INST TECNOL ALIMENTOS, BR-13073001 CAMPINAS, BRAZIL
关键词：
D O I：
10.1016/0021-9673(94)00752-U
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ;
081704 ;
摘要：
To better evaluate the provitamin A content of foodstuffs, an HPLC separation of the geometric isomers of beta-carotene was developed. Of the several. different stationary phases studied, the best separations of all-trans-, beta-cis- and 13-cis-beta-carotene were obtained with calcium hydroxide columns using isocratic elution with n-hexane, isooctane or mixtures of these solvents. When xanthophyll pigments are also present, a gradient elution with acetone in isooctane is required. These techniques were used to determine the provitamin A content of squash, peaches, dende oil (palm tree oil) and orange juice.
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页码：289 / 294
页数：6
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