SEQUENCES OF 2 PORCINE GLUTAMIC-ACID DECARBOXYLASES (65-KDA AND 67-KDA GAD)

被引：11

作者：

SUZUKI, R ^{[1
]}

ASAMI, N ^{[1
]}

AMANN, E ^{[1
]}

WAGATSUMA, M ^{[1
]}

机构：

[1] HOECHST JAPAN LTD, DRUG DISCOVERY RES LABS, KAWAGOE, SAITAMA 35011, JAPAN

来源：

GENE | 1995年 / 152卷 / 02期

关键词：

AMINO-ACID SEQUENCE COMPARISON; POLYMERASE CHAIN REACTION; RAPID AMPLIFICATION OF CDNA ENDS; CAT; RAT;

D O I：

10.1016/0378-1119(94)00713-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The cDNAs encoding the porcine 65- and 67-kDa glutamic acid decarboxylases (GAD(65) and GAD(67), respectively) were cloned by the PCR method. The 2246-nucleotide (nt) GAD(65) cDNA contained an open reading frame (ORF) coding for a protein of 585 amino acids (aa), and the 3262-nt GAD(67) cDNA contained an ORF coding for a protein of 594 aa. A remarkable conservation was shown when the deduced aa sequences of porcine GAD(65) and GAD(67) were compared with those of other mammalian species (human, cat and rat). Porcine GAD(65) is 96% identical to human and rat GAD(65), and porcine GAD(67) is more than 95% identical to human, cat and rat GAD(67) at the aa level.

引用

页码：257 / 260

页数：4

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