The steady-state kinetics of oxidation of rat liver NADPH:cytochrome P450 reductase (EC 1.6.2.4) by quinones, aromatic nitrocompounds, ferricyanide, Fe(EDTA)(-), and cytochrome c has been studied. The logarithms fo bimolecular rate constants of reduction (k(cat)/K-m) of quinones and nitrocompounds increase with the increase in their single-electronreduction potential (E(7)(1)), reaching a maximum value at E(7)(1) > -0.15 V. The reactivities of nitroaromatics are about by an order of magnitude lower than the reactivities of quinones. For a series of nitroaromatics including the compounds with previously undetermined E(7)(1) values, an orthogonality was found between their reactivities toward cytochrome P450 reductase, flavocytochrome b(2) (EC 1.1.2.3), and the NADPH:adrenodoxin reductase (EC 1.18.1.2)-adrenodoxin system. This indicates the absence of significant specific interactions during these reactions. The effects of ionic strength on reaction kinetics and the character of inhibition by a product of reaction, NADP(+), are in accordance with the reduction of oxidants at the negatively charged site in the surroundings of FMN of P450 reductase. Quinones inactivate oxidized reductase modifying the NADP(H) binding site. The redox cycling of quinones markedly slows the inactivation. The kinetic data presented are consistent with an outer-sphere electron transfer mechanism. The analysis of kinetics of reduction of cytochrome c, ferricyanide, and Fe(EDTA)(-) using the model of Mauk et al. (A. G. Mauk, R. A. Scott, and H. B. Gray (1980) J. Am. Chem. Soc. 102, 4360-4363) gives calculated distances of FMN from the surface of protein globule, 0.33-0.63 nm. The data from nitroreductase reactions of cytochrome P450 reductase, flavocytochrome b(2), and adrenodoxin were used for approximate evaluation of previously unknown E(7)(1) of nitrocompounds. (C) 1994 Academic Press, Inc.
