OPTICAL RESOLUTION OF CHLORPHENIRAMINE BY CYCLODEXTRIN ADDED CAPILLARY ZONE ELECTROPHORESIS AND CYCLODEXTRIN MODIFIED MICELLAR ELECTROKINETIC CHROMATOGRAPHY

Optical resolution of RS-chlorpheniramine by cyclodextrin (CD) added capillary zone electrophoresis (CZE) and cyclodextrin modified micellar electrokinetic chromatography (CD-MEKC) was investigated. In CD added CZE, optical resolution was achieved with beta-CD - urea solutions (pH 2.5 - 3.0) with and without methanol. In this system, gamma-CD was not effective to achieve enantiomeric separation. In CD-MEKC, similar resolution as in the CD added CZE systems was obtained with sodium dodecyl sulfate (SDS) - beta-CD - urea solutions (pH 3.0).